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Bearded dragon embryos become females either through sex chromosomes or hot temperatures

Ancient cellular processes are likely involved in temperature-dependent sex reversals

PLOS

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IMAGE: Native to the arid landscapes of Australia, the centra bearded dragon (Pogona vitticeps) is a fascinating species. It has genetic sex determination, but when incubated at high temperatures, genetic males... view more 

Credit: Whiteley SL et al., 2021, PLOS Genetics

Bearded dragon embryos can use two different sets of genes to become a female lizard--one activated by the sex chromosomes and the other activated by high temperatures during development. Sarah Whiteley and Arthur Georges of the University of Canberra report these new findings April 15th in the journal PLOS Genetics.

In many reptiles and fish, the sex of a developing embryo depends on the temperature of the surrounding environment. This phenomenon, called temperature-dependent sex determination, was discovered in the 1960s, but the molecular details of how it happens have eluded scientists despite half a century of intensive research. Researchers investigated the biochemical pathways required to make a female in the new study by studying this phenomenon in bearded dragons. Male bearded dragons have ZZ sex chromosomes, while females have ZW sex chromosomes. However, hot temperatures can override ZZ sex chromosomes, causing a male lizard to develop as a female.

Whiteley and Georges compared which genes were turned on during development in bearded dragons with ZW chromosomes compared to ZZ animals exposed to high temperatures. They discovered that initially, different sets of developmental genes are active in the two types of females, but that ultimately the pathways converge to produce ovaries. The findings support recent research proposing that ancient signaling processes inside the cell help translate high temperatures into a sex reversal.

The new study is the first to show that there are two ways to produce an ovary in the bearded dragon and bringing us closer to understanding how temperature determines sex. The study also identifies several candidate genes potentially involved in temperature-dependent sex determination. These findings lay the foundation for future experiments to tease out each gene's role in sensing temperature and directing sexual development.

Whiteley adds, ""The most exciting component of this work is the discovery that the mechanism involves ubiquitous and highly conserved cellular processes, signaling pathways and epigenetic processes of chromatin modification. This new knowledge is bringing us closer to understanding how temperature determines sex, so it is a very exciting time to be in biology."

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Peer-reviewed; Experimental study; Animals

In your coverage please use this URL to provide access to the freely available article in PLOS Genetics: http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1009465

Citation: Whiteley SL, Holleley CE, Wagner S, Blackburn J, Deveson IW, Marshall Graves JA, et al. (2021) Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination. PLoS Genet 17(4): e1009465. https://doi.org/10.1371/journal.pgen.1009465

Funding: This work was supported by a Discovery Grant from the Australian Research Council (DP170101147) awarded to AG (lead), CEH and JMG https://www.arc.gov.au/. SLW was supported by a CSIRO Research Plus Postgraduate Award https://www.csiro.au/en/Careers/Studentships/Postgraduate-scholarships and a Research Training Scholarship https://www.education.gov.au/research-training-program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

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